Guegan, J.P., Vaultier, M., and Ibora, J.L. Ionic liquids as refolding additives: N′-alkyl and N′-(w-hydroxyalkyl) N-methylimidazolium chloride. Protein renaturation by the liquid organic salt ethylammonium nitrate. Cadena, C., Zhao, Q., Snurr, and R.Q., Maginn, E.J. Holbrey, J.D., McMath, J.E., and Soper, A.K. The effects of ionic liquids on protein crystallization and X-ray diffraction resolution. Fry, E.H., Abad-Zapatero, C., and Chi, M.L. Judge, R.A., Takahashi, S., Longnecker, K.L. Ethylammonium nitrate: A protein crystallization reagent. Garlitz, J.A., Summers, C.A., Flowers, R.A. Advanced protein crystallization using water-soluble ionic liquids as crystallization additives. Protein crystallization using room temperature ionic liquids. Pusey, M.L., Paley, M.S., Turner, M.B., and Rogers, R.D. Ionic liquid Screen contains 24 unique reagents, 0.5 milliliter each.ġ. The Ionic Liquid Screen contains a set of 24 water soluble ionic liquids that comprise different cation (imidazolium, phosphonium, ammonium) and anion (borate, halides, sulfates, acetates, sulfonates, nitrates) structures for a diverse ionic liquid additive screen for use in improving the crystallization behavior and X-ray diffraction resolution of proteins.Įach of the 24 ionic liquids are preformulated in deionized water and sterile filtered using a 0.2 micron filter. Variation of the anion and the cation as well as the utilization of both soft (formate and acetate) and hard anions (nitrate) in the Ionic Liquid Screen reagents provides an additional dimension for evaluating the effects on ionic liquids on the solubility and crystallization of proteins. 13 Many ionic liquids are also organics acids and have ionic character in addition to the hydrophobic behavior, which makes them unique and useful solvents in protein chemistry. Some ionic liquids, such as ethylammonium nitrate have water-like characteristics, including the capacity for hydrogen bonding and the promotion of micelle formation by some surfactants. 9-11 The inclusion of the ionic liquid 1-n-Butyl-3-methylimidazolium tetrafluoroborate (included in this kit) improved the thermal stability and solubility of integral membrane proteins for membrane proteomics study. 7-8 Ionic liquids have been reported to stabilize protein activity and structure. Ionic liquids have been demonstrated to suppress protein aggregation and significantly increase protein folding yields. 1 These organic salts generally consist of combinations of organic cations and either an organic or inorganic anion. Ionic liquids are often chaotropic, composed of low symmetry ions with charge delocalization and weak intermolecular interactions. 5-6 Ionic liquids can participate in ionic, hydrophobic and hydrogen bond interactions. Ionic liquids can demonstrate a degree of localized structuring about each ion compared to materials composed of disassociated ions, setting them apart from salt solutions. Ionic liquids are soluble in a variety of organic and inorganic reagents and can be highly water soluble. They are thermally stable, nonflammable and demonstrate very low vapor pressure. Ionic liquids are organic salts with melting points below 100☌elsius. 25 Ionic liquids have been used as additives to produce crystals in reagents that had previously not resulted in crystallization and results suggest ionic liquids may be applicable for the solubilization and crystallization of membrane proteins. 1-4 The inclusion of ionic liquids in crystallization experiments has been reported to lead to less crystal polymorphism as well as less precipitation at higher precipitant concentrations. Ionic liquids have been found effective as additives in protein crystallization, with different ionic liquids used to increase crystallization rates and crystal size.
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